Nitrocellulose Membrane NC - NuSep
These transfer membranes consist of pure nitrocellulose medium with a high protein binding capacity. They are therefore more robust and durable than other nitrocellulose membranes and do not tear during transfer.
Thanks to the properties of this material, sharper images with little to no distortion are produced.
Applications
- Transfer of protein from SDS-PAGE gels to membrane in preparation for western blotting
- Transfer of a wide range of protein molecular weights and nucleic acids >500 bp (0.45 µm) / <500 bp (0.2 µm)
- Transfer of nucleic acids in preparation for northern or Southern blotting
- Immunodetection of target proteins and probe hybridization of nucleic acids
NuSep Nitrocellulose Membranes NC
NuSep’s 0.45 µm pore-size nitrocellulose membrane is dense 100% nitrocellulose. Pure nitrocellulose is the most frequently used membrane for the transfer of both proteins and nucleic acids. It is a proven medium for western, northern, and Southern blotting that produces excellent signal-to-noise results.
NuSep nitrocellulose is easy to handle, wets readily with water-based solutions, and resists the brittleness usually associated with nitrocellulose. The 0.45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers.
The 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins: the smaller pore size minimizes protein sample loss due to transfer through the membrane.
Specifications
- Compatible with all standard immunoblotting and nucleic acid detection methods
- Provides superior binding capacity without background interference
- Pore size produces excellent signal-to-noise blotting results
- Available in three formats:
- Convenient precut 0.45 µm/0.2 µm membrane/blot absorbent filter paper sandwiches
- Packs of precut membrane-only sheets
- Economical rolls
| Description | Order No. |
|---|---|
| Transfer Membranes NC 0.45 µm (8 x 8 cm) 10 pieces/pack | DBM-NIM-02 |
| Transfer Membranes NC 0.2 µm (8 x 8 cm) 15 pieces/pack | DBM-NIM-03 |
| Transfer Membranes NC 0.45 µm (8 x 10 cm) 15 pieces/pack | DBM-NIM-04 |
| Transfer Membranes NC 0.45 µm (3 x 8 cm) 10 pieces/pack | RB-500 |
| Transfer Membranes NC 0.2 µm (8 x 8 cm) 10 pieces/pack | RB-600 |
| Transfer Membranes NC 0.45 µm (8 x 8 cm) 10 pieces/pack | RB-700 |