Fluorometer EzCube
EzCube Fluorometer features triple fluorescent channels and offers high-sensitivity and specific quantification of nucleic acids and proteins.
It accurately measures low concentration samples as small as 1 µl, making it ideal for demanding applications, including NGS.
- Enhanced sensitivity detection down to 10 pg/μl
- Triple fluorescent channels for diverse applications
- Compatible with EzQuant and standard test kits.
EzCube Fluorometer with Three Fluorescent Channels
Combined with dedicated EzQuant quantification assays, an intuitive touchscreen and USB connectivity, EzCube greatly simplifies your precise quantification processes.
Specifications
- Enhanced Accuracy and Sensitivity:
Quantify low concentrations of DNA, RNA and protein with just 1 µl in seconds, more sensitive than UV absorbance measurements. - Three Fluoresent Channels:
Expand your application range with triple-channel detection. - Easy Operation:
Seamlessly control your workflow with an intuitive 7-inch touchscreen and a built-in reagent calculator. - Flexible Data Management:
USB connectivity for easy data export. - High Compatibility with Quantification Assays:
EzCube fluorometer works with EzQuant quantification assays and other commonly used options.
- 1X dsDNA HS (High-Sensitivity)
- ssDNA
- RNA HS (High-Sensitivity) - Triple-Channel Detection:
Light sources
- Blue LED (max 480 nm)
- Red LED (max 630 nm)
- Green LED (max 535 nm)
Quantification and Quality Control
Accurate cfDNA quantification is key to any successful NGS workflow. This article highlights common pitfalls in the pre-analytical phase and shows how the EzCube Fluorometer´s high sensitivity and specificity help improve sample quality. It also offers practical tips on using the EzCube and EzDrop Micro-Volume Spectrophotometer together for better cfDNA QC.
Critical Considerations for cfDNA Analysis in NGS Applications:
Quantification and Quality Control: Critical Considerations for cfDNA Analysis in NGS Applications-Blue-Ray Biotech
| Dynamic range | 5 orders of magnitude |
| Light sources | Blue LED (max 480 nm) Red LED (max 630 nm) Green LED (max 535 nm) |
| Excitation filters | Blue (430 – 495 nm) Red (600 – 645 nm) Green (490 - 535 nm) |
| Emission filters | Blue (510 – 580 nm) Red (665 – 720 nm) Green (564 – 650 nm) |
| Detectors | Photodiode, measurement capability 320 -1100 nm |
| Tube type | 0.5 ml thin wall Polypropylene PCR tubes |
| Operating system | Custom Linux based OS |
| Display | 7" touch screen, 1,280*800 TFT-LCD |
| Connectivity | USB-A port x2 (data output) |
| Dimensions (W x D x H) | 196 mm x 248 mm x 68 mm |
| Weight | 1 kg |
| Glove compatibility | All common lab gloves |
| Internal storage | 32 GB Flash Memory |
| Operating voltage | Input: AC 100 – 240 V, 50/60 Hz Output: DC 12 V, 3 A |